Thp1 ifn
WebDec 1, 2010 · Apoptosis of THP-1 cells with IFN-γ treatment and Mycobacterium bovis infection is linked to decreased CFU. (a) Apoptosis of IFN-γ-treated THP-1 cells infected with M. bovis (MOI: 10) increased from 4.3±0.6% to 36.5±3.5% during the course of the infection. WebNational Center for Biotechnology Information
Thp1 ifn
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WebJul 14, 2014 · The THP1 cell line is one of the most widely used cell lines for investigations of the function and differentiation of monocytes and Mφs in response to various inflammatory mediators, such as IFN ... WebTHP1-Blue® ISG cells were specifically designed for monitoring the interferon signaling pathway in a physiologically relevant cell line. THP1-Blue® were derived from the human …
Web1 hour ago · Background: Detection of viruses by host pattern recognition receptors induces the expression of type I interferon (IFN) and IFN-stimulated genes (ISGs), which suppress viral replication. Retroviruses such as HIV-1 are subject to sensing by both RNA and DNA sensors, and whether there are any particular features of the viral genome or reverse … WebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. …
WebFigure 1 IFN-γ and LPS cooperatively induce CCL2 in monocytic cells/macrophages. THP-1 monocytic cells, primary monocytes, and THP-1-derived macrophages were treated for … WebMay 20, 2024 · THP1 is a human monocytic cell line derived from an acute monocytic leukemia patient and has been routinely used to study the cGAS/STING pathway as they …
WebTHP1-Lucia™ ISG cells express the secreted luciferase (Lucia) reporter gene under the control of an IRF-inducible promoter. This composite promoter is comprised of five IFN …
WebThese results are consistent with preferential inhibition of these genes, mediated by STAT2 T404 phosphorylation in response to cGAMP and cdi-GMP in THP1 cells (SI Appendix, Fig. S5C). In summary, the phosphorylation of T404 of STAT2 increases its affinity for STING, which inhibits STING-dependent IRF3 activation, resulting in less IFN production. pumpkin apple aztec candle supplyWebThese cells were generated from the human THP1-Dual™ monocytic cell line through a biallelic knockout (KO) of the TRIF gene. They also feature two inducible reporter genes, … secaucus town poolWebOct 14, 2024 · IFN-γ signalling is linked to regional neuronal vulnerability in Parkinson’s ... 2 mM GlutaMAX, and 10% FBS. THP-1 cells were differentiated into macrophages with 25 ng/mL PMA (Sigma) for ... secaucus tx plumbersWebSep 15, 1992 · We have studied two distinct pathways in the differentiation of a human monocytic cell line, THP-1: one being induced by IFN-gamma and the other by 1 alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3). In THP-1 cells, IFN-gamma induces cell surface expression of HLA-DR and CD54 and production of IL-1 beta, TNF-alpha, and IL-6. secaucus vacation rentalsWebInterferon gamma (IFN-gamma) is the most potent known lymphokine for activating macrophages and has been shown to induce expression of HLA-DR in THP-1 cells, a … secaucus universityWebIFN-g-primed THP-1 cells produced higher levels of IL-8 on stimulation with LPS than non-primed cells and the level correlated with duration of priming up to 24 h, although the level of IL-8 induced was most comparable to that induced by co-stimulation with LPS and IFN-g. Unstimulated THP-1 cells were shown by flow cytometry to be pumpkin angel food cake recipeWebNov 17, 2024 · When PMA-treated THP-1 cells were incubated with IFN-λ4 for 48 h, no effect was observed on TNF-α and IL-6 cytokine secretion (Fig. 1B), suggesting that the recombinant protein was free from any endotoxin contamination. Flow cytometry analysis revealed that PMA-treated THP-1 cells 52 had increased IFN-LR1 expression (Fig. 1C). secaucus train to penn station