Web小鼠精胺氧化酶基因SMO启动子的结构和功能研究.pdf WebT4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation and cloning.
T4 RNA Ligase - Promega
WebT4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme. WebT4 DNA Ligase Buffer Pack 1.5ml Designed for use with T4 DNA Ligase to catalyze the joining of two cohesive- or blunt-ended strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides. Formulation: 300mM Tris-HCl (pH 7.8), 100mM MgCl 2, 100mM DTT, 10mM ATP. Specifications What's in the box? SDS Search for SDS es 20 lbs battery
Which ligase should I use? NEB
WebT4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates for this enzyme. WebFAQ. Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single ... WebSep 12, 2016 · The internal T7 promoter of pCR XL TOPO was removed using deletion PCR followed by T4 DNA ligase-T4 polynucleotide kinase-DpnI (NEB, USA) treatment prior to transformation into XL10-GOLD ultracompetent cells (Agilent Technologies, USA). The resulting pT7-EV71 infectious clone was subjected to full-genome sequencing. ... fingers and toes locking up