WebChromatin immunoprecipitation, or ChIP, is an antibody-based technology used to selectively enrich specific DNA-binding proteins along with their DNA targets. ChIP is used to investigate a particular protein-DNA interaction, several protein-DNA interactions, or interactions across the whole genome or a subset of genes. WebApr 30, 2024 · For DSS crosslink assays, the cells or proteoliposomes were suspended in PBS with the indicated concentration of DSS at room temperature for 30 min. The reaction was quenched with 20 mM Tris followed by sample preparation for immunoblot as described before ( Ge et al., 2008 , Ge et al., 2011 ).
Methodology to Quantify Collagen Subtypes and Crosslinks: …
WebJul 1, 2024 · STAT3 protein crosslink assay. To detect the effect of compound 24 on the dimerization of STAT3 protein, we used crosslinker disuccinimidyl suberate (DSS) to crosslink STAT3 protein. DSS can bind with the primary amines in the side chain of lysine residues and the N-terminus of proteins. It thus fixed the STAT3 dimer and assured that … WebNational Center for Biotechnology Information jcpenney playtex bra 4695 online
DSS Homobifunctional Crosslinker Reagent Cyanagen
http://www.proteochem.com/protocols/DSP-Product-Information-Sheet.pdf WebMay 30, 2024 · The chromatin immunoprecipitation (ChIP) assay has recently been exploited as a powerful and versatile technique for probing protein-DNA interactions within the chromatin environment. In this … WebThe neutral comet assay has been employed for DNA double-strand breaks, while techniques using DNA repair enzymes to cleave specific adducts, UvrABC for ultraviolet radiation induced adducts, for example, have also been described. Here, we describe a modified version of the comet assay for the measurement of interstrand crosslinks (ICLs). lsl shares